An aliquot of spermatozoa retrieved from the epididymis or the testis was washed three times in phosphate buffered saline (pH 7.2) and centrifuged at 650 g for 10 min. The sediment was then fixed in ...

Chromosome sorting from fluid suspensions of metaphase chromosomes using a fluorescence-activated cell sorter has been used for a number of years to produce chromosome-specific genomic libraries and ...

WALNUT CREEK, CA-- Four years after publicly revealing the official draft human genetic sequence, researchers have reached the halfway point in dotting the i's and crossing the t's of the genetic ...

Karyotype analysis includes complete chromosome analysis of at least twenty metaphases with at least two cut karyotypes. The presence or absence of an acquired clonal chromosomal abnormality may aid ...

The Cytogenetics Laboratory performs chromosome analysis, fluorescence in situ hybridization (FISH), and array comparative genomic hybridization (array CGH) on cells prepared from a wide variety of ...

The Y chromosome is perhaps the most puzzling part of the human genome. Associated with male development, it is chock-full of repetitive and inverted stretches of DNA, a hurdle that makes it ...

Pt. A. Gene cloning -- cDNA cloning method using stable expression in mammalian cells / Toru Miki and Stuart A. Aaronson -- Normalization and selection with short-fragment cDNAs / Satish Parimoo, ...

Now that certain human disorders have been linked with chromosome abnormalities, it is desirable to examine large numbers of cells for such abnormalities. A computer regime has been devised for the ...

Computational methods used to fill in missing pixels in low-quality images or video also can help scientists provide missing information for how DNA is organized in the cell, computational biologists ...

Chromosomes are thread-like structures comprising DNA that are present inside the nucleus of every cell in the body. Specific segments of DNA are called genes. Every chromosome contains many genes, ...

“There’s nothing like a dame,” sang the Navymen in South Pacific. Not so, say physiologists. There are people who are something like a dame but are really men, or even a combination of male and female ...

Researchers at the Francis Crick Institute, working with the University of Oxford, University of York, and Oxford Archaeology, have developed a new technique to measure the number of chromosomes in ...