Real-time PCR plays a foundational role in gene expression studies, disease analysis, and molecular diagnostics. However, success depends on precise reaction setup, optimized assays, and accurate ...
In a recent study published in Lancet Microbe, researchers compare the potential of real-time reverse transcriptase-polymerase chain reaction (RT-PCR) genotyping assays with whole-genome sequencing ...
How it Works: Real Time PCR Brendan Maher The instrumentation is basic: a thermal cycler for amplification, a light source for excitation of fluorescent probes (see chemistries below), a camera for ...
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