Cell or tissue lysates are arrayed on nitrocellulose-coated slides (Grace Bio-labs, Bend, OR) in triplicate using the Aushon 2470 Arrayer (Aushon BioSystems, Billerica, MA) (technical replicates).
Western blotting is a staple of molecular biology, enabling researchers to identify protein biomarkers and signaling changes that drive various diseases. However, meeting the increasingly stringent ...